Tested Applications
| Positive WB detected in | rat liver tissue, LNCaP cells, mouse lung tissue, HepG2 cells, K-562 cells, mouse liver tissue, rabbit liver tissue |
| Positive IHC detected in | human thyroid cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67905-1-Ig targets METTL7A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.
| Tested Reactivity | human, mouse, rat, rabbit |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29986 Product name: Recombinant human METTL7A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-244 aa of BC008180 Sequence: MELTIFILRLAIYILTFPLYLLNFLGLWSWICKKWFPYFLVRFTVIYNEQMASKKRELFSNLQEFAGPSGKLSLLEVGCGTGANFKFYPPGCRVTCIDPNPNFEKFLIKSIAENRHLQFERFVVAAGENMHQVADGSVDVVVCTLVLCSVKNQERILREVCRVLRPGGAFYFMEHVAAECSTWNYFWQQVLDPAWHLLFDGCNLTRESWKALERASFSKLKLQHIQAPLSWELVRPHIYGYAVK Predict reactive species |
| Full Name | methyltransferase like 7A |
| Calculated Molecular Weight | 28 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC008180 |
| Gene Symbol | METTL7A |
| Gene ID (NCBI) | 25840 |
| RRID | AB_2918661 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9H8H3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
methyltransferase like 7A (METTL7A) is a 244 amino acid protein which belongs to the methyltransferase super family. Methyltransferase can transfer a methyl group from a donor to an acceptor. Methylation may be linked to cancer development as methylation of tumor suppressor genes promotes tumorgenesis and metastasis.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for METTL7A antibody 67905-1-Ig | Download protocol |
| WB protocol for METTL7A antibody 67905-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The METTL7A Monoclonal antibody (catalog number 67905-1-Ig) has 3 citations. These appear in the journals International Journal of Oral Science, Cell Signaling, and iScience. The associated research fields include bone biology and osteogenic differentiation of orofacial mesenchymal stem cells, acute myeloid leukemia progression and drug resistance, and lung adenocarcinoma with macrophage polarization. The antibody was applied in Western blot and immunohistochemistry for human samples.
| Species | Application | Title |
|---|---|---|
Int J Oral Sci METTL7A-mediated m6A modification of corin reverses bisphosphonates-impaired osteogenic differentiation of orofacial BMSCs
| ||
Cell Signal USP9X promotes acute myeloid leukemia progression and adriamycin resistance by regulating METTL7A deubiquitination.
| ||
iScience TMT1A inhibits lung adenocarcinoma progression by suppressing M2 macrophage polarization. |
