MIER2 Recombinant antibody

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  WB analysis using 84216-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84216-1-RR (MIER2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 84216-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MIER2 antibody (84216-1-RR, Clone: 241458D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84216-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84216-1-RR against Human MIER2 were performed. The affinity constant is below 1 pM.

MIER2 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84216-1-RR

Clone No.241458D2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Mi-er2, Mesoderm induction early response protein 2, KIAA1193, 241458D2

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Tested Applications

• Positive WB detected in: U2OS cells, HEK-293 cells, Jurkat cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84216-1-RR targets MIER2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MIER2 fusion protein Ag11491
• Full Name: mesoderm induction early response 1, family member 2
• Calculated Molecular Weight: 545 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC028203
• Gene Symbol: MIER2
• Gene ID (NCBI): 54531
• RRID: AB_3671772
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N344
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mesoderm induction early response 2 (MIER2) is a member of the MIER family of proteins, which is relatively conservative in evolution and includes three members: MIER1, MIER2, and MIER3. The MIER family is known to contribute to histone deacetylase (HDAC) activity and actively participate in histone deacetylation (PMID: 28046085; PMID: 38702028). In renal cell carcinoma, MIER2 represses PGC1A expression by recruiting HDAC1 to deacetylate P53 (PMID: 28046085).