MIS12 Polyclonal antibody, PBS Only

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• MIS12 Polyclonal antibody, PBS Only (19820-1-PBS)

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  WB analysis using 19820-1-AP (same clone as 19820-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19820-1-AP (MIS12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19820-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 19820-1-AP (same clone as 19820-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19820-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 19820-1-AP (same clone as 19820-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19820-1-AP (MIS12 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19820-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 19820-1-AP (same clone as 19820-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MIS12 antibody (19820-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 19820-1-PBS in a different storage buffer formulation.

MIS12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 19820-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

hMis12, KNTC2AP, MTW1, Protein MIS12 homolog

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Product Information

19820-1-PBS targets MIS12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13870

  Product name: Recombinant human MIS12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-205 aa of BC000229

  Sequence: MSVDPMTYEAQFFGFTPQTCMLRIYIAFQDYLFEVMQAVEQVILKKLDGIPDCDISPVQIRKCTEKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPEDKCKETPYSEEDFQHLQKEIEQLQEKYKTELCTKQALLAELEEQKIVQAKLKQTLTFFDELHNVGRDHGTSDFRESLVSLVQNSRKLQNIRDNVEKESKRLKIS

• Full Name: MIS12, MIND kinetochore complex component, homolog (yeast)
• Calculated Molecular Weight: 205 aa, 24 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC000229
• Gene Symbol: MIS12
• Gene ID (NCBI): 79003
• RRID: AB_3669315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H081
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The MIS12 complex, composed of the Mis12, Pmf1, Nsl1 and Dsn1 subunits, is a highly conserved kinetochore protein that interacts with the Ndc80 and Knl1 complexes, forming the KMN network (PMID: 32341029). MIS12 is a part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. MIS12 is essential for proper kinetochore microtubule attachments (PubMed:23891108)