MIS12 Monoclonal antibody

MIS12 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G5F6

Cat no : 67475-1-Ig

Synonyms

2510025F08Rik, hMis12, KNTC2AP, MIS12, MTW1, Protein MIS12 homolog

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Validation Data Gallery

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  WB analysis using 67475-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67475-1-Ig (MIS12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67475-1-Ig targets MIS12 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MIS12 fusion protein Ag16826
• Full Name: MIS12, MIND kinetochore complex component, homolog (yeast)
• Calculated Molecular Weight: 205 aa, 24 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC000229
• Gene Symbol: MIS12
• Gene ID (NCBI): 79003
• RRID: AB_2882702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.