CoraLite® Plus 488-conjugated MIS12 Monoclonal antibody

MIS12 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2G5F6

Cat no : CL488-67475

Synonyms

2510025F08Rik, hMis12, KNTC2AP, MIS12, MTW1, Protein MIS12 homolog

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Validation Data Gallery

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  FC experiment of HeLa using CL488-67475

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MIS12 (CL488-67475, Clone:2G5F6) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67475 targets MIS12 in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MIS12 fusion protein Ag16826
• Full Name: MIS12, MIND kinetochore complex component, homolog (yeast)
• Calculated Molecular Weight: 205 aa, 24 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC000229
• Gene Symbol: MIS12
• Gene ID (NCBI): 79003
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.