CoraLite® Plus 488-conjugated MIS12 Monoclonal antibody

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  WB analysis using CL488-67475

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-67475 (MIS12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using CL488-67475

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MIS12 (CL488-67475, Clone:2G5F6) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

MIS12 Monoclonal Antibody for WB, FC (Intra)

Cat No. CL488-67475

Clone No.2G5F6

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, FC (Intra)

Synonyms

Protein MIS12 homolog, MTW1, KNTC2AP, hMis12, 2G5F6

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67475 targets MIS12 in WB, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16826

  Product name: Recombinant human MIS12 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-205 aa of BC000229

  Sequence: MSVDPMTYEAQFFGFTPQTCMLRIYIAFQDYLFEVMQAVEQVILKKLDGIPDCDISPVQIRKCTEKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPEDKCKETPYSEEDFQHLQKEIEQLQEKYKTELCTKQALLAELEEQKIVQAKLKQTLTFFDELHNVGRDHGTSDFRESLVSLVQNSRKLQNIRDNVEKESKRLKIS

• Full Name: MIS12, MIND kinetochore complex component, homolog (yeast)
• Calculated Molecular Weight: 205 aa, 24 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC000229
• Gene Symbol: MIS12
• Gene ID (NCBI): 79003
• RRID: AB_2934544
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H081
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.