Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, MOLT-4 cells, U2OS cells, NIH/3T3 cells, HSC-T6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
88031-1-RR targets MITD1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag10998 Product name: Recombinant human MITD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-249 aa of BC018453 Sequence: MAKSGLRQDPQSTAAATVLKRAVELDSESRYPQALVCYQEGIDLLLQVLKGTKDNTKRCNLREKISKYMDRAENIKKYLDQEKEDGKYHKQIKIEENATGFSYESLFREYLNETVTEVWIEDPYIRHTHQLYNFLRFCEMLIKRPCKVKTIHLLTSLDEGIEQVQQSRGLQEIEESLRSHGVLLEVQYSSSIHDREIRFNNGWMIKIGRGLDYFKKPQSRFSLGYCDFDLRPCHETTVDIFHKKHTKNI Predict reactive species |
| Full Name | MIT, microtubule interacting and transport, domain containing 1 |
| Calculated Molecular Weight | 249 aa, 29 kDa |
| Observed Molecular Weight | 29 kDa |
| GenBank Accession Number | BC018453 |
| Gene Symbol | MITD1 |
| Gene ID (NCBI) | 129531 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8WV92 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MITD1 (Microtubule Interacting and Trafficking Domain Containing 1) has a molecular weight of approximately 29.3 kDa and consists of 249 amino acids. Functionally, MITD1 regulates ESCRT-III activity and is required for efficient abscission at the end of cytokinesis. It functions as a homodimer, with its N-terminal MIT domain binding to a subset of ESCRT-III subunits and its C-terminal unanticipated phospholipase D-like (PLD) domain binding to membranes. Depletion of MITD1 causes cytokinetic defects, including midbody destabilization and abscission failure. Beyond its role in cell division, MITD1 has recently been identified as a brain-specific interferon-inducible factor that inhibits flavivirus replication.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MITD1 antibody 88031-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
