ChromoTek MK2-Trap Agarose, kit

ChromoTek’s MK2-Trap Agarose kit are affinity beads for IP of MK2-fusion proteins. It comprises a MK2 Nanobody/ VHH conjugated to agarose beads.

Specificity

MK2

Reactivity

Human, Mouse, Hamster

Applications

IP, CoIP

Conjugate

Agarose

Type

Nanobody

Cat no : mtak

Synonyms

MK2-Trap, MK2, MAPKAPK2, MAPKAP kinase 2, MAPKAPK 2



Product Information

ChromoTek’s MK2-Trap Agarose kit are affinity beads for IP of MK2-fusion proteins. It comprises a MK2 Nanobody/ VHH conjugated to agarose beads.

DescriptionThe MK2-Trap Agarose Kit contains MK2-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of MK2.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains on the SDS-PAGE

• Stable under harsh washing conditions

• Short incubation time (1 h at 4°C)

ApplicationsIP, CoIP
Specificity/TargetMK2 (MAP kinase-activated protein kinase 2, MAPKAP-K2)
Tested ReactivityHuman, Mouse, Hamster
Posttranslational modifications: MK2-Trap recognizes unphosphorylated MK2 and Phospho-MK2 (Thr222). Specificity on Phospho-MK2 (Thr334) was not tested.
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Type Nanobody
ClassRecombinant
HostAlpaca
Compatibility with mass spectrometryThe MK2-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631376
Storage Buffer20% ethanol
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
MK2-Trap Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution

Other Formats

MK2-Trap Agarose