ChromoTek MK2-Trap Agarose

ChromoTek’s MK2-Trap Agarose are affinity beads for IP of MK2-fusion proteins. It comprises a MK2 Nanobody/ VHH conjugated to agarose beads.

Specificity

MK2

Applications

IP, CoIP

Conjugate

Agarose

Cat no : mta

Synonyms

MK2-Trap, MK2, MAPKAPK2, MAPKAP kinase 2, MAPKAPK 2



Product Information

ChromoTek’s MK2-Trap Agarose are affinity beads for IP of MK2-fusion proteins. It comprises a MK2 Nanobody/ VHH conjugated to agarose beads.

ApplicationsIP, CoIP
ClonalityMonoclonal
HostAlpaca
Specificity/TargetMK2 (MAP kinase-activated protein kinase 2, MAPKAP-K2)
Species-Reactivity: Tested on human, mouse, hamster
Posttranslational modifications: MK2-Trap recognizes unphosphorylated MK2 and Phospho-MK2 (Thr222). Specificity on Phospho-MK2 (Thr334) was not tested.
Coupled VHHRecombinant, monoclonal anti-MK2 single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Publications

ApplicationTitle
IP

Cell Rep

Alternative Translation Initiation Generates a Functionally Distinct Isoform of the Stress-Activated Protein Kinase MK2.

Authors - Philipp Trulley
IP

Proc Natl Acad Sci U S A

MK2 degradation as a sensor of signal intensity that controls stress-induced cell fate.

Authors - Nuria Gutierrez-Prat