MKNK2 Polyclonal antibody, PBS Only

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• MKNK2 Polyclonal antibody, PBS Only (17354-1-PBS)

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  WB analysis of mouse spleen using 17354-1-AP (same clone as 17354-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17354-1-AP (MKNK2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17354-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 17354-1-AP (same clone as 17354-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17354-1-AP (MKNK2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17354-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 17354-1-AP (same clone as 17354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17354-1-AP (MKNK2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17354-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 17354-1-AP (same clone as 17354-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17354-1-AP (MKNK2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17354-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 17354-1-AP (same clone as 17354-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MKNK2 antibody (17354-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17354-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

MKNK2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 17354-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

MNK2, MAPK signal-integrating kinase 2, MAP kinase-interacting serine/threonine-protein kinase 2, MAP kinase signal-integrating kinase 2, GPRK7

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Product Information

17354-1-PBS targets MKNK2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10546

  Product name: Recombinant human MKNK2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-158 aa of BC018345

  Sequence: MAPEVVEAFSEEASIYDKRCDLWSLGVILYILLSGYPPFVGRCGSDCGWDRGEACPACQNMLFESIQEGKYEFPDKDWAHISCAAKDLISKLLVRDAKQRLSAAQVLQHPWVQGCAPENTLPTPMVLQRWDSHFLLPPHPCRIHVRPGGLVRTVTVNE

• Full Name: MAP kinase interacting serine/threonine kinase 2
• Calculated Molecular Weight: 465 aa, 52 kDa
• Observed Molecular Weight: 47-52 kDa
• GenBank Accession Number: BC018345
• Gene Symbol: MKNK2
• Gene ID (NCBI): 2872
• RRID: AB_2878395
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HBH9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MKNK2(MAP kinase-interacting serine/threonine-protein kinase 2) is also named as GPRK7, MNK2 and belongs to the CAMK Ser/Thr protein kinase family. It has a conserved C-terminal Erk-interacting domain, a catalytic domain with homology to the calcium/calmodulin-dependent family of kinases, and putative MAP kinase phosphorylation sites located within the T loop of the kinase domain(PMID:9155017). The breast cancer cells express either an uncharacterized form of MNK2 or a cross-reacting MNK2-like protein with the band of 72-75 kDa by western blot(PMID:17130135). MKNK2 has 2 isoforms with the molecular mass of 52 kDa and 47 kDa.