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MLF1IP Polyclonal antibody, PBS Only (13186-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 13186-1-AP (same clone as 13186-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.
WB analysis using 13186-1-AP (same clone as 13186-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.
WB analysis using 13186-1-AP (same clone as 13186-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13186-1-AP (MLF1IP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 13186-1-AP (same clone as 13186-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13186-1-AP (MLF1IP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 13186-1-AP (same clone as 13186-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13186-1-AP (MLF1IP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 13186-1-AP (same clone as 13186-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 13186-1-AP (same clone as 13186-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 13186-1-AP (same clone as 13186-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 13186-1-AP (same clone as 13186-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13186-1-AP (MLF1IP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 13186-1-AP (same clone as 13186-1-PBS)
Immunofluorescent analysis of HepG2 cells using 13186-1-AP (MLF1IP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13186-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

MLF1IP Polyclonal antibody, PBS Only

Name: MLF1IP Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 13186-1-PBS

MLF1IP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 13186-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CENP 50, CENP U, CENP U(50), CENP50, CENP-50

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

13186-1-PBS targets MLF1IP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3858 Product name: Recombinant human MLF1IP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 243-418 aa of BC031520 Sequence: MKTSDIKELNIVLPEFEKTHLEHQQRIESKVCKAAIATFYVNVKEQFIKMLKESQMLTNLKRKNAKMISDIEKKRQRMIEVQDELLRLEPQLKQLQTKYDELKERKSSLRNAAYFLSNLKQLYQDYSDVQAQEPNVKETYDSSSLPALLFKARTLLGAESHLRNINHQLEKLLDQG Predict reactive species
Full Name	MLF1 interacting protein
Calculated Molecular Weight	418 aa, 48 kDa
Observed Molecular Weight	46-50 kDa, 60 kDa
GenBank Accession Number	BC031520
Gene Symbol	MLF1IP
Gene ID (NCBI)	79682
RRID	AB_10642941
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q71F23
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

MLF1IP, also named as CENPU, ICEN24, KLIP1 and PBIP1, belongs to the CENP-U/AME1 family. MLF1IP plays an important role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. Moreover, MLF1IP has been shown to be involved in tumorigenesis in several types of cancer including glioblastoma, prostate cancer and luminal breast cancer (PMID: 27378428). MLF1IP has 3 isoforms with the molecular mass of 21, 38 and 47 kDa.