FC experiment of Transfected HEK-293T using 98361-3-RR
1x10^6 HEK-293T cells (left) or MMP8 transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Rat MMP8 Rabbit RecAb (98361-3-RR, Clone: 242473B12) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HEK-293T cells (left) or MMP8 transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Rat MMP8 Rabbit RecAb (98361-3-RR, Clone: 242473B12) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
FC experiment of Transfected HEK-293T using 98361-3-RR
1x10^6 HEK-293T cells (left) or MMP8 transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Rat MMP8 Rabbit RecAb (98361-3-RR, Clone:242473B12) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HEK-293T cells (left) or MMP8 transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Rat MMP8 Rabbit RecAb (98361-3-RR, Clone:242473B12) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
98361-3-RR targets MMP8 in FC (Intra) applications and shows reactivity with rat samples.
Store at 2 - 8°C. Stable for one year after shipment.
Background Information
MMP8(Matrix metalloproteinase-8) an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells(PMID:15367487).
FC experiment of Transfected HEK-293T using 98361-3-RR
1x10^6 HEK-293T cells (left) or MMP8 transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Rat MMP8 Rabbit RecAb (98361-3-RR, Clone: 242473B12) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
FC experiment of Transfected HEK-293T using 98361-3-RR
1x10^6 HEK-293T cells (left) or MMP8 transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Rat MMP8 Rabbit RecAb (98361-3-RR, Clone:242473B12) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
MMP8 Recombinant antibody
Catalog Number
98361-3-RR
Citations
-
Dilutions
FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension
Applications
FC (Intra)
Reactivity
rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
