MRPL38 Polyclonal antibody

MRPL38 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15913-1-AP

Synonyms

MRP L38, MRP L3, Large ribosomal subunit protein mL38, L38mt, KIAA1863

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Validation Data Gallery

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  WB analysis using 15913-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15913-1-AP (MRPL38 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15913-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15913-1-AP (MRPL38 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15913-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15913-1-AP (MRPL38 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15913-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15913-1-AP (MRPL38 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15913-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15913-1-AP (MRPL38 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HEK-293 using 15913-1-AP

  1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug MRPL38 Polyclonal antibody (15913-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human liver tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

15913-1-AP targets MRPL38 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRPL38 fusion protein Ag8706
• Full Name: mitochondrial ribosomal protein L38
• Calculated Molecular Weight: 346 aa, 41 kDa
• Observed Molecular Weight: 37-45 kDa
• GenBank Accession Number: BC013311
• Gene Symbol: MRPL38
• Gene ID (NCBI): 64978
• RRID: AB_2146047
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling; Authors - Jana Key
rat	WB	Cell Rep; Small RNAs derived from tRNA fragmentation regulate the functional maturation of neonatal β cells.; Authors - Mustafa Bilal Bayazit
	IHC	J Toxicol Sci; Aberrant epigenetic gene regulation in hippocampal neurogenesis of mouse offspring following maternal exposure to 3,3'-iminodipropionitrile.; Authors - Takeshi Tanaka