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MSH6 Monoclonal antibody
MSH6 Monoclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2E10B2
Cat no : 66172-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HEK-293 |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
IHC | See 4 publications below |
Product Information
66172-1-Ig targets MSH6 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | MSH6 fusion protein Ag12645 |
Full Name | mutS homolog 6 (E. coli) |
Calculated molecular weight | 153 kDa |
Observed molecular weight | 160 kDa |
GenBank accession number | BC004246 |
Gene symbol | MSH6 |
Gene ID (NCBI) | 2956 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for MSH6 antibody 66172-1-Ig | Download protocol |
IHC protocol for MSH6 antibody 66172-1-Ig | Download protocol |
IF protocol for MSH6 antibody 66172-1-Ig | Download protocol |
IP protocol for MSH6 antibody 66172-1-Ig | Download protocol |
FC protocol for MSH6 antibody 66172-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Med Chem CJ2: A Novel Potent Platinum(IV) Prodrug Enhances Chemo-Immunotherapy by Facilitating PD-L1 Degradation in the Cytoplasm and Cytomembrane | ||
Surgery Mismatch repair status as a beneficial predictor of fluorouracil-based adjuvant chemotherapy for pancreatic cancer. | ||
J Surg Oncol Clinicopathological and survival characteristic of mismatch repair status in ovarian clear cell carcinoma. | ||
Oncol Lett HDAC10 expression is associated with DNA mismatch repair gene and is a predictor of good prognosis in colon carcinoma. |