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MSI2 Polyclonal antibody, PBS Only (10770-1-PBS)

Filter: AllWBIPIHC

WB analysis of PC-12 using 10770-1-AP (same clone as 10770-1-PBS)
PC-12 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.
WB analysis of COLO 320 using 10770-1-AP (same clone as 10770-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.
WB analysis of C6 using 10770-1-AP (same clone as 10770-1-PBS)
C6 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.
WB analysis of C6 using 10770-1-AP (same clone as 10770-1-PBS)
C6 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 10770-1-AP (same clone as 10770-1-PBS)
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.
WB analysis of PC-12 using 10770-1-AP (same clone as 10770-1-PBS)
PC-12 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.
WB analysis of PC-12 using 10770-1-AP (same clone as 10770-1-PBS)
PC-12 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 10770-1-AP (same clone as 10770-1-PBS)
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 10770-1-AP (same clone as 10770-1-PBS)
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.
IP experiment of MCF-7 using 10770-1-AP (same clone as 10770-1-PBS)
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10770-1-AP (MSI2 antibody) at dilution of 1:500. This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 10770-1-AP (same clone as 10770-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 10770-1-AP (same clone as 10770-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 10770-1-AP (same clone as 10770-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 10770-1-AP (same clone as 10770-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 10770-1-AP (same clone as 10770-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 10770-1-AP (same clone as 10770-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10770-1-AP (MSI2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10770-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

MSI2 Polyclonal antibody, PBS Only

Name: MSI2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 10770-1-PBS

MSI2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 10770-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Musashi-2, MSI2H, Musashi 2, RNA-binding protein Musashi homolog 2

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

10770-1-PBS targets MSI2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1213 Product name: Recombinant human Musashi-2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC001526 Sequence: MEANGSQGTSGSANDSQHDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADPASVDKVLGQPHHELDSKTIDPKVAFPRRAQPKMVTRTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVMFPPGTRGRARGLPYTMDAFMLGMGMLGYPNFVATYGRGYPGFAPSYGYQFPGFPAAAYGPVAAAAVAAARGSGSNPARPGGFPGANSPGPVADLYGPASQDSGVGNYISAASPQPGSGFGHGIAGPLIATAFTNGYH Predict reactive species
Full Name	musashi homolog 2 (Drosophila)
Calculated Molecular Weight	35 kDa
Observed Molecular Weight	37-40 kDa
GenBank Accession Number	BC001526
Gene Symbol	MSI2
Gene ID (NCBI)	124540
RRID	AB_2147935
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96DH6
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

MSI2 is an RNA-binding protein that regulates the expression of target mRNAs at the translation level. It binds to RNA via 2 ribonucleoprotein-type RNA recognition motifs in their N-termini, to consensus motifs in mRNAs to inhibit transcription. It may play a role in the proliferation and maintenance of stem cells in the central nervous system. MSI2 can regulate normal hematopoiesis and promote aggressive myeloid leukemia