MTA3 Polyclonal antibody, PBS Only

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• MTA3 Polyclonal antibody, PBS Only (14682-1-PBS)

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  WB analysis using 14682-1-AP (same clone as 14682-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14682-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 14682-1-AP (same clone as 14682-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14682-1-PBS in a different storage buffer formulation.

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  IP experiment of Raji using 14682-1-AP (same clone as 14682-1-PBS)

  IP result of anti-MTA3 (IP:14682-1-AP, 4ug; Detection:14682-1-AP 1:3000) with Raji cells lysate 2840 ug. This data was developed using the same antibody clone with 14682-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14682-1-AP (same clone as 14682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14682-1-AP (MTA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14682-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14682-1-AP (same clone as 14682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14682-1-AP (MTA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14682-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 14682-1-AP (same clone as 14682-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (14682-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14682-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 14682-1-AP (same clone as 14682-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (14682-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 14682-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

MTA3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 14682-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Metastasis-associated protein MTA3, MTA 3, mta1

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Product Information

14682-1-PBS targets MTA3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6400

  Product name: Recombinant human MTA3 protein

  Source: e coli.-derived, PKG

  Tag: GST

  Domain: 1-343 aa of BC053631

  Sequence: MAANMYRVGDYVYFENSSSNPYLIRRIEELNKTASGNVEAKVVCFYRRRDISNTLIMLADKHAKEIEEESETTVEADLTDKQKHQLKHRELFLSRQYESLPATHIRGKCSVALLNETESVLSYLDKEDTFFYSLVYDPSLKTLLADKGEIRVGPRYQADIPEMLLEGESDEREQSKLEVKVWDPNSPLTDRQIDQFLVVARAVGTFARALDCSSSVRQPSLHMSAAAASRDITLFHAMDTLYRHSYDLSSAISVLVPLGGPVLCRDEMEEWSASEASLFEEALEKYGKDFNDIRQDFLPWKSLTSIIEYYYMWKTTDRYVQQKRLKAAEAESKLKQVYIPTYS

• Full Name: metastasis associated 1 family, member 3
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 62 kDa, 68 kDa
• GenBank Accession Number: BC053631
• Gene Symbol: MTA3
• Gene ID (NCBI): 57504
• RRID: AB_2298003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BTC8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.