Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat testis tissue |
| Positive IF/ICC detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16888-1-AP targets MTCH2 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10399 Product name: Recombinant human MTCH2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 31-178 aa of BC000875 Sequence: GYEPLPPTIGRNIFGRQVCQLPGLFSYAQHIASIDGRRGLFTGLTPRLCSGVLGTVVHGKVLQHYQESDKGEELGPGNVQKEVSSSFDHVIKETTREMIARSAATLITHPFHVITLRSMVQFIGRESKYCGLCDSIITIYREEGILGF Predict reactive species |
| Full Name | mitochondrial carrier homolog 2 (C. elegans) |
| Calculated Molecular Weight | 303 aa, 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC000875 |
| Gene Symbol | MTCH2 |
| Gene ID (NCBI) | 23788 |
| RRID | AB_2266733 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6C9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MTCH2 antibody 16888-1-AP | Download protocol |
| IHC protocol for MTCH2 antibody 16888-1-AP | Download protocol |
| IP protocol for MTCH2 antibody 16888-1-AP | Download protocol |
| WB protocol for MTCH2 antibody 16888-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
16888-1-AP is an MTCH2 antibody with 29 total citations. Publications appear in journals including Nature Communications, Molecular Cell, EMBO Journal, Science Advances, Cell Death & Disease, Journal of Cell Biology, iScience, FASEB Journal, and American Journal of Human Genetics. Research fields span mitochondrial biology and dynamics, various cancers (glioma, gastric, ovarian, breast, colorectal), neurodegeneration (Parkinson's, Alzheimer's), metabolic regulation, and mitochondrial outer membrane protein function.
| Species | Application | Title |
|---|---|---|
Nat Commun RHOA regulates mitochondria-ER contact sites through modulation of the VAPB/PTPIP51 tether. | ||
Mol Cell Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology
| ||
Adv Sci (Weinh) Neuronal FGF13 Inhibits Mitochondria-Derived Damage Signals to Prevent Neuroinflammation and Neurodegeneration in a Mouse Model of Parkinson's Disease | ||
Sci Adv ARMC1 partitions between distinct complexes and assembles MIRO with MTFR to control mitochondrial distribution | ||
Cell Death Dis MTCH2 regulates NRF2-mediated RRM1 expression to promote melanoma proliferation and dacarbazine insensitivity
| ||
Int J Mol Med Dihydroartemisinin inhibits the viability of cervical cancer cells by upregulating caveolin 1 and mitochondrial carrier homolog 2: Involvement of p53 activation and NAD(P)H:quinone oxidoreductase 1 downregulation. |
Reviews
What customers say
The single review for this product is highly positive, rated 5 stars. The user reported using it for both Western Blot and Immunofluorescence, noting that the WB band was strong and clear and that the IFA image binding was excellent. Overall, the feedback highlights reliable performance and high-quality results across both applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Xianhe (Verified Customer) (06-23-2022) | WB band is strong and clear, IFA image binding great.
|
