CoraLite® Plus 488-conjugated MTMR9 Recombinant monoclonal antibody

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  IF Staining of HeLa using CL488-83694-4

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 MTMR9 antibody (CL488-83694-4, Clone: 240167G7 ) at dilution of 1:200, CL594-Phalloidin (red).

MTMR9 Recombinant Antibody for IF/ICC

Cat No. CL488-83694-4

Clone No.240167G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

myotubularin related protein 9, MTMR8, LIP STYX, Inactive phosphatidylinositol 3-phosphatase 9, C8orf9

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83694-4 targets MTMR9 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag34134

  Product name: Recombinant human MTMR9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 424-549 aa of BC022003

  Sequence: MLFEHAYASQFGTFLGNNESERCKLKLQQKTMSLWSWVNQPSELSKFTNPLFEANNLVIWPSVAPQSLPLWEGIFLRWNRSSKYLDEAYEEMVNIIEYNKELQAKVNILRRQLAELETEDGMQESP

• Full Name: myotubularin related protein 9
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC022003
• Gene Symbol: MTMR9
• Gene ID (NCBI): 66036
• RRID: AB_3673290
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96QG7
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MTMR9 (Myotubularin-related protein 9) acts as an adapter for myotubularin-related phosphatases (PMID: 19038970; 22647598). MTMR9 can increase lipid phosphatase MTMR6 catalytic activity, specifically towards phosphatidylinositol 3,5-bisphosphate and MTMR6 binding affinity for phosphorylated phosphatidylinositols (PMID: 19038970; 22647598). It can also increase MTMR8 catalytic activity towards phosphatidylinositol 3-phosphate and stabilize MTMR8 protein levels (PMID: 22647598).