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MUC16/CA125 Polyclonal antibody, PBS Only (20077-1-PBS)

Filter: AllWBIPIHC

WB analysis of HeLa using 20077-1-AP (same clone as 20077-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 20077-1-AP (MUC16,CA125 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 20077-1-AP (same clone as 20077-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 20077-1-AP (MUC16,CA125 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 20077-1-AP (same clone as 20077-1-PBS)
IP result of anti-MUC16,CA125 (IP:20077-1-AP, 4ug; Detection:20077-1-AP 1:600) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 20077-1-AP (same clone as 20077-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20077-1-AP (MUC16,CA125 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 20077-1-AP (same clone as 20077-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20077-1-AP (MUC16,CA125 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20077-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

MUC16/CA125 Polyclonal antibody, PBS Only

Name: MUC16/CA125 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 20077-1-PBS

MUC16/CA125 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 20077-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

MUC16, MUC16,CA125, MUC-16, MUC 16, CA125

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

20077-1-PBS targets MUC16/CA125 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	mucin 16, cell surface associated
Calculated Molecular Weight	1519 kDa
Observed Molecular Weight	90-100 kDa
GenBank Accession Number	NM_024690
Gene Symbol	MUC16
Gene ID (NCBI)	94025
RRID	AB_10695048
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WXI7
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

MUC16, also named as CA125, is thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. It is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller "subunits" being described by some investigators. The antibody recognizes the C-term of MUC16.