Methylmalonyl Coenzyme A mutase/MUT Polyclonal antibody, PBS Only

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• Methylmalonyl Coenzyme A mutase/MUT Polyclonal antibody, PBS Only (17034-1-PBS)

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  WB analysis using 17034-1-AP (same clone as 17034-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17034-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 17034-1-AP (same clone as 17034-1-PBS)

  IP result of anti-Methylmalonyl Coenzyme A mutase/MUT (IP:17034-1-AP, 4ug; Detection:17034-1-AP 1:500) with HepG2 cells lysate 1800ug. This data was developed using the same antibody clone with 17034-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 17034-1-AP (same clone as 17034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17034-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 17034-1-AP (same clone as 17034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17034-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 17034-1-AP (same clone as 17034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17034-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 17034-1-AP (same clone as 17034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17034-1-AP (Methylmalonyl Coenzyme A mutase/MUT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17034-1-PBS in a different storage buffer formulation.

Methylmalonyl Coenzyme A mutase/MUT Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 17034-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

MUT, EC:5.4.99.2, MCM, Methylmalonyl CoA isomerase, Methylmalonyl Coenzyme A mutase

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Product Information

17034-1-PBS targets Methylmalonyl Coenzyme A mutase/MUT in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10523

  Product name: Recombinant human MUT protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 401-750 aa of BC016282

  Sequence: SARIARNTQIIIQEESGIPKVADPWGGSYMMECLTNDVYDAALKLINEIEEMGGMAKAVAEGIPKLRIEECAARRQARIDSGSEVIVGVNKYQLEKEDTVEVLAIDNTSVRNRQIEKLKKIKSSRDQALAERCLAALTECAASGDGNILALAVDASRARCTVGEITDALKKVFGEHKANDRMVSGAYRQEFGESKEITSAIKRVHKFMEREGRRPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPREVAQQAVDADVHAVGVSTLAAGHKTLVPELIKELNSLGRPDILVMCGGVIPPQDYEFLFEVGVSNVFGPGTRIPKAAVQVLDDIEKCLEKKQQSV

• Full Name: methylmalonyl Coenzyme A mutase
• Calculated Molecular Weight: 750 aa, 83 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC016282
• Gene Symbol: MUT
• Gene ID (NCBI): 4594
• RRID: AB_2147263
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P22033
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Methylmalonyl Coenzyme A mutase (MUT) is an enzyme that plays a crucial role in the metabolism of certain amino acids and fatty acids (PMID: 15647267). Mutations in the MUT gene can lead to methylmalonic acidemia, a metabolic disorder characterized by the accumulation of toxic compounds such as methylmalonyl-CoA and propionyl-CoA (PMID: 30428564), resulting in life-threatening metabolic acidosis, respiratory distress, neurological impairment, hyperammonemia, and ketosis (PMID: 32679819). MUT is essential for maintaining normal metabolic processes and its dysfunction can have significant health implications, highlighting its importance in both basic metabolism and clinical medicine (PMID: 23041189).