Tested Applications
| Positive WB detected in | IFN alpha treated A549 cells, mouse lung tissue |
| Positive IP detected in | IFN alpha treated A549 cells |
| Positive IHC detected in | human tonsillitis tissue, human colon cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13750-1-AP targets MX1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, canine, monkey, chicken, hamster, pavo cristatus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4692 Product name: Recombinant human MX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 312-662 aa of BC032602 Sequence: LLEEGKATVPCLAEKLTSELITHICKSLPLLENQIKETHQRITEELQKYGVDIPEDENEKMFFLIDKINAFNQDITALMQGEETVGEEDIRLFTRLRHEFHKWSTIIENNFQEGHKILSRKIQKFENQYRGRELPGFVNYRTFETIVKQQIKALEEPAVDMLHTVTDMVRLAFTDVSIKNFEEFFNLHRTAKSKIEDIRAEQEREGEKLIRLHFQMEQIVYCQDQVYRGALQKVREKELEEEKKKKSWDFGAFQSSSATDSSMEEIFQHLMAYHQEASKRISSHIPLIIQFFMLQTYGQQLQKAMLQLLQDKDTYSWLLKERSDTSDKRKFLKERLARLTQARRRLAQFPG Predict reactive species |
| Full Name | Myxovirus resistance protein 1 |
| Calculated Molecular Weight | 662 aa, 76 kDa |
| Observed Molecular Weight | 76 kDa |
| GenBank Accession Number | BC032602 |
| Gene Symbol | MX1 |
| Gene ID (NCBI) | 4599 |
| RRID | AB_2266768 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20591 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN. This antibody can recognize the endogenous Mx1 protein in several tissues like lung and spleen.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for MX1 antibody 13750-1-AP | Download protocol |
| IF protocol for MX1 antibody 13750-1-AP | Download protocol |
| IHC protocol for MX1 antibody 13750-1-AP | Download protocol |
| IP protocol for MX1 antibody 13750-1-AP | Download protocol |
| WB protocol for MX1 antibody 13750-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MX1 Polyclonal antibody (Cat# 13750-1-AP) has 125 citations across journals including Nature, Cell, Immunity, Nature Communications, PLoS Pathogens, Journal of Virology, Journal of Immunology, Cell Host & Microbe, and Annals of the Rheumatic Diseases. Research fields span antiviral innate immunity, interferon signaling, viral infections (influenza, hepatitis B, HIV, SARS-CoV-2), autoimmune diseases (lupus, Sjögren's syndrome), and oncology (oncolytic virotherapy, head and neck cancer, breast cancer).
| Species | Application | Title |
|---|---|---|
Immunity Virus-induced deconjugation of ISG15 dysregulates innate immunity and cellular metabolism. | ||
Ann Rheum Dis Peptidylarginine deiminase inhibition disrupts NET formation and protects against kidney, skin and vascular disease in lupus-prone MRL/lpr mice. | ||
Reviews
What customers say
The single review reports successful use of this antibody for Western Blot at a 1:500 dilution. The user tested human fibroblasts with and without interferon treatment and observed a single specific band only in the interferon-treated samples, indicating clear detection of the target upon stimulation. The reviewer rated the product 5 out of 5, reflecting satisfaction with its specificity and performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hannah (Verified Customer) (09-21-2020) | Used for western blot on human fibroblasts -/+ interferon treatment, detected single band only in IFN treated samples.
 |
