Validation Data Gallery
|Positive WB detected in
|A549 cells, mouse lung tissue
|Positive IP detected in
|Positive IHC detected in
|human tonsillitis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:500-1:1000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
13750-1-AP targets MX1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, Chicken, monkey, hamster, Pavo cristatus, pig, canine, macaque
|Host / Isotype
|Rabbit / IgG
|MX1 fusion protein Ag4692
|Myxovirus resistance protein 1
|Calculated molecular weight
|662 aa, 76 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN. This antibody can recognize the endogenous Mx1 protein in several tissues like lung and spleen.
IFITM3 restricts the morbidity and mortality associated with influenza.
Cell Host Microbe
NRAV, a long noncoding RNA, modulates antiviral responses through suppression of interferon-stimulated gene transcription.
Large-scale and high-resolution mass spectrometry-based proteomics profiling defines molecular subtypes of esophageal cancer for therapeutic targeting.
MxA inhibits hepatitis B virus replication by interaction with hepatitis B core antigen.
Repeated semen exposure decreases cervicovaginal SIVmac251 infection in rhesus macaques.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hannah (Verified Customer) (09-21-2020)
Used for western blot on human fibroblasts -/+ interferon treatment, detected single band only in IFN treated samples.