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MX1 Polyclonal antibody

MX1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (6)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13750-1-AP

Synonyms

IFI 78K, IFI78, MX, MX1, MxA, Myxovirus resistance protein 1



Tested Applications

Positive WB detected inA549 cells, mouse lung tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and N/A for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13750-1-AP targets MX1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, canine, Chicken, macaque, monkey, Pavo cristatus, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MX1 fusion protein Ag4692
Full Name Myxovirus resistance protein 1
Calculated molecular weight 662 aa, 76 kDa
Observed molecular weight 76 kDa
GenBank accession numberBC032602
Gene symbol MX1
Gene ID (NCBI) 4599
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN. This antibody can recognize the endogenous Mx1 protein in several tissues like lung and spleen.

Protocols

Product Specific Protocols
WB protocol for MX1 antibody 13750-1-APDownload protocol
IHC protocol for MX1 antibody 13750-1-APDownload protocol
IF protocol for MX1 antibody 13750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

IFITM3 restricts the morbidity and mortality associated with influenza.

Authors - Everitt AR
humanWB

Cell Host Microbe

NRAV, a long noncoding RNA, modulates antiviral responses through suppression of interferon-stimulated gene transcription.

Authors - Jing Ouyang
human

Nat Commun

Large-scale and high-resolution mass spectrometry-based proteomics profiling defines molecular subtypes of esophageal cancer for therapeutic targeting.

Authors - Wei Liu
humanWB

Hepatology

MxA inhibits hepatitis B virus replication by interaction with hepatitis B core antigen.

Authors - Li Ning N
macaqueIHC

Nat Commun

Repeated semen exposure decreases cervicovaginal SIVmac251 infection in rhesus macaques.

Authors - Shaheed A Abdulhaqq
humanWB

J Hepatol

PMID: 25481566

Authors - Shi Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hannah (Verified Customer) (09-21-2020)

Used for western blot on human fibroblasts -/+ interferon treatment, detected single band only in IFN treated samples.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/500
MX1 Antibody Western Blot validation (1/500 dilution) in (Cat no:13750-1-AP)