|Positive WB detected in||A549 cells, mouse lung tissue|
|Positive IP detected in||A549 cells|
|Positive IHC detected in||human tonsillitis tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A549 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and N/A for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13750-1-AP targets MX1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||Chicken, dog, human, Indian peafowls, Macaca mulata, monkey, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MX1 fusion protein Ag4692|
|Full Name||Myxovirus resistance protein 1|
|Calculated molecular weight||662 aa, 76 kDa|
|Observed molecular weight||76 kDa|
|GenBank accession number||BC032602|
|Gene ID (NCBI)||4599|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN. This antibody can recognize the endogenous Mx1 protein in several tissues like lung and spleen.
The large GTPase Mx1 binds Kif5B for cargo transport along microtubules.
Br J Cancer
Interferon-alpha promotes immunosuppression through IFNAR1/STAT1 signalling in head and neck squamous cell carcinoma.
J Med Virol
Induction of interleukin 6 impairs the anti-HBV efficiency of IFN-α in human hepatocytes through up-regulation of SOCS3.
Cell Stress Chaperones
Molecular cloning and transcriptional regulation of Indian peafowl (Pavo cristatus) IFN-α gene.
The histone chaperone HIRA promotes the induction of host innate immune defences in response to HSV-1 infection.
SFTSV Infection Induces BAK/BAX-Dependent Mitochondrial DNA Release to Trigger NLRP3 Inflammasome Activation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hannah (Verified Customer) (09-21-2020)
Used for western blot on human fibroblasts -/+ interferon treatment, detected single band only in IFN treated samples.