Tested Applications
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-13750 targets MX1 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4692 Product name: Recombinant human MX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 312-662 aa of BC032602 Sequence: LLEEGKATVPCLAEKLTSELITHICKSLPLLENQIKETHQRITEELQKYGVDIPEDENEKMFFLIDKINAFNQDITALMQGEETVGEEDIRLFTRLRHEFHKWSTIIENNFQEGHKILSRKIQKFENQYRGRELPGFVNYRTFETIVKQQIKALEEPAVDMLHTVTDMVRLAFTDVSIKNFEEFFNLHRTAKSKIEDIRAEQEREGEKLIRLHFQMEQIVYCQDQVYRGALQKVREKELEEEKKKKSWDFGAFQSSSATDSSMEEIFQHLMAYHQEASKRISSHIPLIIQFFMLQTYGQQLQKAMLQLLQDKDTYSWLLKERSDTSDKRKFLKERLARLTQARRRLAQFPG Predict reactive species |
| Full Name | Myxovirus resistance protein 1 |
| Calculated Molecular Weight | 662 aa, 76 kDa |
| Observed Molecular Weight | 76 kDa |
| GenBank Accession Number | BC032602 |
| Gene Symbol | MX1 |
| Gene ID (NCBI) | 4599 |
| RRID | AB_2919077 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20591 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN. This antibody can recognize the endogenous Mx1 protein in several tissues like lung and spleen.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 MX1 antibody CL488-13750 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MX1 Polyclonal antibody (CL488-13750) has 2 citations. They appear in Small (2024, IF 11.8), covering synthetic lipopeptide-based influenza inhibition and immuno-stimulating effects in mouse, and Acta Biomaterialia (2025, IF 10.4), addressing polyethyleneimine-modified eggshell membrane for bone and periosteum regeneration in rat using IF. The associated research fields span virology/immunology and tissue engineering/biomaterials.
| Species | Application | Title |
|---|---|---|
Small A Synthetic Multivalent Lipopeptide Derived from Pam3CSK4 with Irreversible Influenza Inhibition and Immuno-Stimulating Effects | ||
Acta Biomater Polyethyleneimine-modified eggshell membrane for dual bone and periosteum regeneration: A multifunctional approach for guided bone healing |
Reviews
What customers say
The single review for this product is highly positive (5/5). The reviewer used the antibody for flow cytometry and reported that it performs very well even at low concentrations, producing a strong and clean signal. Overall, the feedback highlights excellent sensitivity and signal quality in flow cytometry applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Alexandra (Verified Customer) (03-01-2024) | The antibody works very well, on a low concentration and gives a very nice signal.
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