MXI1 Recombinant antibody

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  WB analysis using 84733-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84733-5-RR (MXI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 84733-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MXI1 Recombinant antibody (84733-5-RR, Clone:242089D2) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84733-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84733-5-RR against Human MXI1 were performed. The affinity constant is below 1 pM.

MXI1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 84733-5-RR

Clone No.242089D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

MAX interactor 1, Max interacting protein 1, MAD2, Class C basic helix-loop-helix protein 11, bHLHc11

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, mouse brain tissue
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84733-5-RR targets MXI1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MXI1 fusion protein Ag3024
• Full Name: MAX interactor 1
• Calculated Molecular Weight: 228 aa, 26 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC012907
• Gene Symbol: MXI1
• Gene ID (NCBI): 4601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P50539
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MXI1 is a transcription factor containing a basic helix-loop-helix leucine zipper (bHLHzip) and belongs to the Myc-Max-Mad transcriptional network. It is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors.