MYCBP Recombinant antibody

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  WB analysis using 85891-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85891-1-RR (MYCBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MYCBP Recombinant Antibody for WB, ELISA

Cat No. 85891-1-RR

Clone No.250129G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

AMY 1, AMY1, AMY-1, Associate of Myc 1, c-Myc-binding protein

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, HEK-293 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85891-1-RR targets MYCBP in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MYCBP fusion protein Ag2651
• Full Name: c-myc binding protein
• Calculated Molecular Weight: 103 aa, 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC008686
• Gene Symbol: MYCBP
• Gene ID (NCBI): 26292
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99417
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYC binding protein(MYCBP), encoded by MYCBP gene, binds to N-terminal region of MYC, and then MYC can activate E box-dependent transcription. Upon increasing expression of MYC , MYCBP translocates into the nucleus in the S phase of the cell cycle. MYCBP may involve in spermatogenesis, since it's found to be interacted with AKAP84/149 in the mitochondria in somatic cells and sperm. It highly expressed in heart, placenta, pancreas,skeletal muscle and kidney, but low in lung. This is a rabbit polyclonal antibody raised against full-length MYCBP of human origin.