MYH7-specific Polyclonal antibody, PBS Only

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• MYH7-specific Polyclonal antibody, PBS Only (22280-1-PBS)

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  WB analysis using 22280-1-AP (same clone as 22280-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22280-1-AP (MYH7-specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 22280-1-AP (same clone as 22280-1-PBS)

  IP result of anti-MYH7-specific (IP:22280-1-AP, 4ug; Detection:22280-1-AP 1:3000) with mouse heart tissue lysate 1840 ug. This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 22280-1-AP (same clone as 22280-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22280-1-AP (MYH7-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 22280-1-AP (same clone as 22280-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22280-1-AP (MYH7-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 22280-1-AP (same clone as 22280-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22280-1-AP (MYH7-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 22280-1-AP (same clone as 22280-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22280-1-AP (MYH7-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 22280-1-AP (same clone as 22280-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22280-1-AP (MYH7-specific antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 22280-1-AP (same clone as 22280-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22280-1-AP (MYH7-specific antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 22280-1-AP (same clone as 22280-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYH7-specific antibody (22280-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 22280-1-AP (same clone as 22280-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using MYH7-specific antibody (22280-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

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  IF Staining of H9C2 using 22280-1-AP (same clone as 22280-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using MYH7-specific antibody (22280-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22280-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

MYH7-specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Cat No. 22280-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Synonyms

MYH7, Myosin 7, MYHCB, MyHC-beta, MyHC-slow

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Product Information

22280-1-PBS targets MYH7-specific in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: myosin, heavy chain 7, cardiac muscle, beta
• Calculated Molecular Weight: 223 kDa
• Observed Molecular Weight: 200-220 kDa
• GenBank Accession Number: NM_000257
• Gene Symbol: MYH7
• Gene ID (NCBI): 4625
• RRID: AB_2736821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P12883
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Myosin is the major contractile protein of all eukaryotic cells, existing as a hexameric molecule composed of two myosin heavy chains (MHCs) that contain the catalytic sites for ATP hydrolysis, and four lower molecular weight myosin light chains. MYH7 encodes the slow/beta-cardiac myosin heavy chain (MHC), which is the major MHC isoform of cardiac ventricles. It is also expressed in slow, oxidative, type 1 muscle fibers of skeletal muscle. Numerous mutations of MYH7 have been linked to familial hypertrophic cardiomyopathy. This antibody can specifically recognize endogenous MYH7, not cross-reacting with other MHCs (PMID: 2523412, 14520662). Due to the production of Heavy Meromyosin (HMM) during proteolysis of myosin, a band around 150 kDa may be detected, which may correspond to the HMM part of MYH7 (PMID: 14613868).