MYPN-Specific Polyclonal antibody, PBS Only

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• MYPN-Specific Polyclonal antibody, PBS Only (16180-1-PBS)

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  WB analysis using 16180-1-AP (same clone as 16180-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16180-1-AP (same clone as 16180-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 16180-1-AP (same clone as 16180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16180-1-AP (MYPN-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 16180-1-AP (same clone as 16180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16180-1-AP (MYPN-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 16180-1-AP (same clone as 16180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 16180-1-AP (same clone as 16180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16180-1-AP (same clone as 16180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16180-1-AP (same clone as 16180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16180-1-AP (same clone as 16180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MYPN-Specific antibody (16180-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 16180-1-PBS in a different storage buffer formulation.

MYPN-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 16180-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

MYPN, MYOP, Myopalladin

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Product Information

16180-1-PBS targets MYPN-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: myopalladin
• Calculated Molecular Weight: 145 kDa
• Observed Molecular Weight: 145 kDa
• GenBank Accession Number: NM_032578
• Gene Symbol: MYPN
• Gene ID (NCBI): 84665
• RRID: AB_2146767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86TC9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MYPN is a component of the sarcomere that tethers nebulin in skeletal muscle and nebulette in cardiac muscle to alpha-actinin at the Z lines (from OMIM). Myopalladin and palladin share C-terminal fragment in sequence. While myopalladin are most abundant in skeletal and cardiac muscle, palladin is ubiquitously expressed in the organs of developing vertebrates. This antibody is specific to myopalladin.