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Mammaglobin A Monoclonal antibody

Mammaglobin A Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF

Conjugate

Unconjugated

CloneNo.

2E5D4

Cat no : 66237-1-Ig

Synonyms

Mammaglobin 1, Mammaglobin A, MGB1, SCGB2A2, UGB2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Mammaglobin A Monoclonal antibody (66237-1-Ig)

    IHC staining of human breast cancer using 66237-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66237-1-Ig (Mammaglobin A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Mammaglobin A Monoclonal antibody (66237-1-Ig)

    IHC staining of human breast cancer using 66237-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66237-1-Ig (Mammaglobin A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Mammaglobin A Monoclonal antibody (66237-1-Ig)

    IHC staining of human breast cancer using 66237-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66237-1-Ig (Mammaglobin A antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Mammaglobin A Monoclonal antibody (66237-1-Ig)

    IHC staining of human breast cancer using 66237-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66237-1-Ig (Mammaglobin A antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Mammaglobin A Monoclonal antibody (66237-1-Ig)

    IHC staining of human breast cancer using 66237-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66237-1-Ig (Mammaglobin A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Mammaglobin A Monoclonal antibody (66237-1-Ig)

    IHC staining of human breast cancer using 66237-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66237-1-Ig (Mammaglobin A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Mammaglobin A Monoclonal antibody (66237-1-Ig)

    IHC staining of human breast cancer using 66237-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66237-1-Ig (Mammaglobin A antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Mammaglobin A Monoclonal antibody (66237-1-Ig)

    IHC staining of human breast cancer using 66237-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66237-1-Ig (Mammaglobin A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using Mammaglobin A Monoclonal antibody (66237-1-Ig)

    IF Staining of human breast cancer using 66237-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Mammaglobin A antibody (66237-1-Ig, Clone: 2E5D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66237-1-Ig targets Mammaglobin A in IHC, IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Mammaglobin A fusion protein Ag22485
Full Name secretoglobin, family 2A, member 2
Calculated Molecular Weight 93 aa, 10 kDa
GenBank Accession NumberBC128252
Gene Symbol SCGB2A2
Gene ID (NCBI) 4250
RRIDAB_2881626
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammaglobin A, also known as SCGB2A2, is a member of the secretoglobin superfamily. Mammaglobin A is a breast cancer-associated antigen almost exclusively over-expressed in primary and metastatic human breast cancers, making it a specific molecular marker and a potential therapeutic target for breast cancer. This monoclonal antibody is raised against full-length of 93-amino acid human mammaglobin A.

Protocols

Product Specific Protocols
IHC protocol for Mammaglobin A antibody 66237-1-IgDownload protocol
IF protocol for Mammaglobin A antibody 66237-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols