Maspin Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• Maspin Polyclonal antibody, PBS Only (11722-1-PBS)

• 

  WB analysis of HepG2 using 11722-1-AP (same clone as 11722-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11722-1-AP (Maspin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 11722-1-AP (same clone as 11722-1-PBS)

  WB result of Maspin antibody (11722-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Maspin transfected HeLa cells. This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 11722-1-AP (same clone as 11722-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 11722-1-AP (Maspin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 11722-1-AP (same clone as 11722-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11722-1-AP (Maspin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11722-1-AP (same clone as 11722-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11722-1-AP (Maspin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bowen disease using 11722-1-AP (same clone as 11722-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11722-1-AP (Maspin antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bowen disease using 11722-1-AP (same clone as 11722-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11722-1-AP (Maspin antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 11722-1-AP (same clone as 11722-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11722-1-AP (Maspin antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 11722-1-AP (same clone as 11722-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11722-1-AP (Maspin antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11722-1-AP (same clone as 11722-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11722-1-AP (Maspin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 11722-1-AP (same clone as 11722-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Maspin antibody (11722-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11722-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Maspin Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 11722-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SERPINB5, Peptidase inhibitor 5, PI 5, PI5, PI-5

---

Product Information

11722-1-PBS targets Maspin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2304

  Product name: Recombinant human SERPINB5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-231 aa of BC020713

  Sequence: MDALQLANSAFAVDLFKQLCEKEPLGNVLFSPICLSTSLSLAQVGAKGDTANEIGQVLHFENVKDVPFGFQTVTSDVNKLSSFYSLKLIKRLYVDKSLNLSTEFISSTKRPYAKELETVDFKDKLEETKGQINNSIKDLTDGHFENILADNSVNDQTKILVVNAAYFVGKWMKKFPESETKECPFRVNKVCGAACSSKRSPIIDVKNDRDRVGHKSIPMRNLRARPAKCLS

• Full Name: serpin peptidase inhibitor, clade B (ovalbumin), member 5
• Calculated Molecular Weight: 375 aa, 42 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC020713
• Gene Symbol: Maspin
• Gene ID (NCBI): 5268
• RRID: AB_10597111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P36952
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SERPINB5, also named PI-5, Maspin, and Serpin B5, belongs to the serpin family and Ov-serpin subfamily. It is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, SERPINB5 exhibits no serine protease inhibitory activity.