Melan-A Recombinant monoclonal antibody

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• Melan-A Recombinant monoclonal antibody (87351-4-RR)

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  WB analysis of SK-MEL-30 using 87351-4-RR

  SK-MEL-30 cells were subjected to SDS PAGE followed by western blot with 87351-4-RR (MLANA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 87351-4-RR

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 87351-4-RR (MLANA antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 87351-4-RR

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 87351-4-RR (MLANA antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Melan-A Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 87351-4-RR

Clone No.252647C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

melan A, MLANA, Antigen LB39 AA, Antigen LB39-AA, Antigen SK29 AA

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Tested Applications

• Positive WB detected in: SK-MEL-30 cells
• Positive IHC detected in: human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87351-4-RR targets Melan-A in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag13346

  Product name: Recombinant human MLANA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-118 aa of BC014423

  Sequence: MPREDAHFIYGYPKKGHGHSYTTAEEAAGIGILTVILGVLLLIGCWYCRRRNGYRALMDKSLHVGTQCALTRRCPQEGFDHRDSKVSLQEKNCEPVVPNAPPAYEKLSAEQSPPPYSP

• Full Name: melan-A
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 13-20 kDa
• GenBank Accession Number: BC014423
• Gene Symbol: MelanA
• Gene ID (NCBI): 2315
• RRID: AB_3745484
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16655
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Melan-A is a melanocyte differentiation antigen, recognized by autologous cytotoxic T lymphocytes. Melan-A is also called MART-1 (melanoma antigen recognized by T cells). The Melan-A/MART-1 gene encodes this protein, 20-22 kDa, associated with endoplasmic reticulum and melanosomes. The function of the protein is unknown. Melan-A, isolated as a melanoma-specific antigen, is a transmembrane protein, which is expressed in skin, retina and the majority of cultured melanocytes as well as in melanomas. Melan A is expressed in more than 85% of melanomas.