Biotin-conjugated Melan-A Recombinant monoclonal antibody

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• Biotin-conjugated Melan-A Recombinant monoclonal antibody (Biotin-87351-4)

Melan-A Uni-rAb^® Recombinant Antibody for IHC

Cat No. Biotin-87351-4 Copied!

Clone No. 252647C9

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC

Synonyms

melan A, MLANA, Antigen LB39 AA, Antigen LB39-AA, Antigen SK29 AA

• 

  IHC staining of human malignant melanoma using Biotin-87351-4

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using Biotin-87351-4 (Melan-A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: human malignant melanoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-87351-4 targets Melan-A in IHC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag13346

  Product name: Recombinant human MLANA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-118 aa of BC014423

  Sequence: MPREDAHFIYGYPKKGHGHSYTTAEEAAGIGILTVILGVLLLIGCWYCRRRNGYRALMDKSLHVGTQCALTRRCPQEGFDHRDSKVSLQEKNCEPVVPNAPPAYEKLSAEQSPPPYSP

• Full Name: melan-A
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 13-20 kDa
• GenBank Accession Number: BC014423
• Gene Symbol: MelanA
• Gene ID (NCBI): 2315
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16655
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Melan-A is a melanocyte differentiation antigen, recognized by autologous cytotoxic T lymphocytes. Melan-A is also called MART-1 (melanoma antigen recognized by T cells). The Melan-A/MART-1 gene encodes this protein, 20-22 kDa, associated with endoplasmic reticulum and melanosomes. The function of the protein is unknown. Melan-A, isolated as a melanoma-specific antigen, is a transmembrane protein, which is expressed in skin, retina and the majority of cultured melanocytes as well as in melanomas. Melan A is expressed in more than 85% of melanomas.