Recombinant Mouse CD300LG protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2731

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Validation Data Gallery

• 

  Purity of Recombinant Mouse CD300LG was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse CD300LG protein Leu19-Ile284 (Accession# Q1ERP8-3) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 52685
• Accession: Q1ERP8-3
• PredictedSize: 54.5 kDa
• SDS-PAGE: 58-100 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD300LG, also known as CD300 molecule-like family member G, is a part of the CD300 antigen-like family. it is known to have at least four isoforms in both species. The immunoglobulin (Ig) V-like domain of CD300LG exhibits approximately 35% identity to those of the polymeric Ig receptor (pIgR) and Fcα/μR. mouse CD300LG proteins are uniquely expressed on capillary endothelium. CD300LG may play a significant role in molecular transport across the capillary endothelium. In addition, CD300LG is capable of bidirectional transcytosis in assays using polarized MDCK epithelial cells.

References:

1. Hiroyuki Takatsu. et al. (2006). Biochem Biophys Res Commun. 15;348(1):183-191.
2. Randall S Davis. et al. (2005). Eur J Immunol. 35(3):674-680.
3. B.J. Green. et al. (1998). Int. Immunol. 10(7):891-899.