Recombinant Mouse Epha6 protein (rFc Tag)(HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90%, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2857

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Validation Data Gallery

• 

  Purity of Recombinant Mouse Epha6 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Mouse Epha6 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90%, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse Epha6 protein Ser28-Gln546 (Accession# Q62413) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 13840
• Accession: Q62413
• PredictedSize: 84.1 kDa
• SDS-PAGE: 85-110 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Epha6 (Eph receptor A6) is a receptor tyrosine kinase that binds to a mixed GPI-anchored heparin-A family ligand residing on neighboring cells, resulting in contact-dependent bidirectional signaling to neighboring cells. Epha6 plays a critical role in brain development and/or behavioral regulation, and the inactivation of its gene leads to impaired learning and memory. Epha6 is associated with a variety of diseases, its overexpression leads to the progression of human prostate cancer, and EphA6 overexpression has been identified as an important independent prognostic indicator in breast cancer.

References:

1. Das G, et al. Cell Biosci. (2016). 6:48.                                                                          
2. Zhou D, et al. Oncol Lett. (2018).15(4):5257-5263. 
3. Li S, et al. Oncotarget. (2015). 6(26):22587-97.