Recombinant Mouse TFPI protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3128

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Validation Data Gallery

• 

  Purity of Recombinant Mouse TFPI was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse TFPI protein Leu29-Lys289 (Accession# O54819-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 21788
• Accession: O54819-1
• PredictedSize: 55.7 kDa
• SDS-PAGE: 60-70 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Tissue Factor Pathway Inhibitor (TFPI), also known as TFPI1, EPI, LACI, or TFI, encodes a Kunitz-type serine protease inhibitor that plays a pivotal role in regulating the blood coagulation process. TFPI reduces the development of atherosclerosis by regulating tissue factor (TF) mediated coagulation pathway. Changes in the expression of tissue factor pathway inhibitor (TFPI), thrombomodulin, selectins, and adhesion molecules in pulmonary endothelial cells as well as complex regulation and interaction of vasoactive substances and growth factors released from endothelium may underlie the mechanisms of pulmonary endothelial dysfunction in COPD.

References:

1. Yuan HQ. et al. (2019). Clin Chim Acta. 491:97-102. 
2. Yang Q. (2008). Curr Drug Metab. 9(7):661-7.