NAP1L1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NAP1L1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 14898-1-AP

Synonyms

Nucleosome assembly protein 1-like 1, NRP, NAP-1-related protein, NAP1L, NAP1

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Validation Data Gallery

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  WB analysis of Jurkat using 14898-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14898-1-AP

  WB result of NAP1L1 antibody (14898-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAP1L1 transfected HeLa cells.

• 

  WB analysis of HEK-293 using 14898-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14898-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14898-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14898-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 14898-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14898-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 14898-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14898-1-AP (NAP1L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 14898-1-AP

  IP result of anti-NAP1L1 (IP:14898-1-AP, 4ug; Detection:14898-1-AP 1:5000) with HEK-293 cells lysate 1440 ug.

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  IHC staining of human testis using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 14898-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14898-1-AP (NAP1L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 14898-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAP1L1 antibody (14898-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  IF Staining of NIH/3T3 using 14898-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using NAP1L1 antibody (14898-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of HeLa using 14898-1-AP

  Immunofluorescent analysis of HepG2 cells, using NAP1L1 antibody 14898-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of NIH/3T3 using 14898-1-AP

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug NAP1L1 Polyclonal antibody (14898-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HT-1080 cells, A431 cells, HEK-293 cells, mouse thymus tissue, HL-60 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, NIH/3T3 cells
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Product Information

14898-1-AP targets NAP1L1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NAP1L1 fusion protein Ag6690
• Full Name: nucleosome assembly protein 1-like 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC002387
• Gene Symbol: NAP1L1
• Gene ID (NCBI): 4673
• RRID: AB_2150696
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; NAP1L1 Functions as a Tumor Promoter via Recruiting Hepatoma-Derived Growth Factor/c-Jun Signal in Hepatocellular Carcinoma.; Authors - Ye-Wei Zhang ; KD Validated
human	WB	Oncotarget; Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.; Authors - Liyuan Zhang
mouse	IF,WB	J Virol; NAP1L1 and NAP1L4 binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein is bivalent and requires phosphorylation.; Authors - Francisco Dominguez
human	WB,IHC,IF	Cancer Cell Int; NAP1L1 interacts with hepatoma-derived growth factor to recruit c-Jun inducing breast cancer growth.; Authors - Shu Liu ; KD Validated
human	WB	J Dermatol Sci; Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells.; Authors - Satoru Mizuhashi
human	WB,IF	Cytoskeleton (Hoboken); NAP1L1 is a novel microtubule-associated protein; Authors - Nannan Peng