Tested Applications
| Positive WB detected in | rat liver tissue |
| Positive IHC detected in | human small intestine tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11410-1-AP targets NAT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1953 Product name: Recombinant human NAT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-290 aa of BC015878 Sequence: MDIEAYFERIGYKNSRNKLDLETLTDILEHQIRAVPFENLNMHCGQAMELGLEAIFDHIVRRNRGGWCLQVNQLLYWALTTIGFQTTMLGGYFYIPPVNKYSTGMVHLLLQVTIDGRNYIVDAGSGSSSQMWQPLELISGKDQPQVPCIFCLTEERGIWYLDQIRREQYITNKEFLNSHLLPKKKHQKIYLFTLEPQTIEDFESMNTYLQTSPTSSFITTSFCSLQTPEGVYCLVGFILTYRKFNYKDNTDLVEFKTLTEEEVEEVLKNIFKISLGRNLVPKPGDGSLTI Predict reactive species |
| Full Name | N-acetyltransferase 2 (arylamine N-acetyltransferase) |
| Calculated Molecular Weight | 33.5 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC015878 |
| Gene Symbol | NAT2 |
| Gene ID (NCBI) | 10 |
| RRID | AB_3085371 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11245 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NAT2 (Arylamine N-acetyltransferase 2) is well-known for its role in the phase II metabolism of xenobiotics and drugs (PMID:36156098). NAT2 and its isozyme, arylamine N-acetyltransferase 1 (NAT1), play key roles in the detoxification of carcinogenic arylamines and xenobiotics by catalyzing acetyl-coenzyme A (CoA)-dependent biotransformation of arylamines to N-arylacetamides. Endogenous NAT2 expression is limited to the liver and small and large intestines, suggesting that NAT2 plays organ-specific roles (PMID:24467436).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for NAT2 antibody 11410-1-AP | Download protocol |
| IHC protocol for NAT2 antibody 11410-1-AP | Download protocol |
| WB protocol for NAT2 antibody 11410-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this antibody is a 5-star rating from a Western Blot application. The user tested it on mouse liver tissue at a 1:4000 dilution and reported a strong signal with clean background, describing it as a very good antibody for WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (04-26-2024) | Very good antibody for WB. Strong signal and clean backgroud.
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