NCAM2 Polyclonal antibody, PBS Only

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• NCAM2 Polyclonal antibody, PBS Only (13850-1-PBS)

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  WB analysis using 13850-1-AP (same clone as 13850-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13850-1-AP (NCAM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13850-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 13850-1-AP (same clone as 13850-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 13850-1-AP (NCAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13850-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 13850-1-AP (same clone as 13850-1-PBS)

  WB result of NCAM2 antibody (13850-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAM2 transfected LNCaP cells. This data was developed using the same antibody clone with 13850-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 13850-1-AP (same clone as 13850-1-PBS)

  IP result of anti-NCAM2 (IP:13850-1-AP, 4ug; Detection:13850-1-AP 1:5000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 13850-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13850-1-AP (same clone as 13850-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13850-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13850-1-AP (same clone as 13850-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13850-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 13850-1-AP (same clone as 13850-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13850-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 13850-1-AP (same clone as 13850-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13850-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NCAM2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 13850-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

N CAM 2, NCAM 2, NCAM-2, NCAM21

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Product Information

13850-1-PBS targets NCAM2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4821

  Product name: Recombinant human NCAM2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 30-351 aa of BC052946

  Sequence: VELSVGESKFFTCTAIGEPESIDWYNPQGEKIISTQRVVVQKEGVRSRLTIYNANIEDAGIYRCQATDAKGQTQEATVVLEIYQKLTFREVVSPQEFKQGEDAEVVCRVSSSPAPAVSWLYHNEEVTTISDNRFAMLANNNLQILNINKSDEGIYRCEGRVEARGEIDFRDIIVIVNVPPAISMPQKSFNATAERGEEMTFSCRASGSPEPAISWFRNGKLIEENEKYILKGSNTELTVRNIINSDGGPYVCRATNKAGEDEKQAFLQVFVQPHIIQLKNETTYENGQVTLVCDAEGEPIPEITWKRAVDGFTFTEGDKSLD

• Full Name: neural cell adhesion molecule 2
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 100-125 kDa
• GenBank Accession Number: BC052946
• Gene Symbol: NCAM2
• Gene ID (NCBI): 4685
• RRID: AB_2237029
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15394
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The mammalian NCAM family has two members, NCAM1 and NCAM2 (PMID: 9729879; 20439718). Neural cell adhesion molecule 2 (NCAM2, also known as NCAM21) is a single-pass type I membrane protein that is highly expressed in the brain, which may play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons or in neurodegenerative diseases such as Alzheimer's disease (PMID: 26611261). NCAM2 has two isoforms: NCAM2.1 bearing transmembrane and cytoplasmic domains and the shorter NCAM2.2 isoform, which is GPI-anchored (PMID: 9334170; 9221781). The molecular weight of NCAM2 is around 125 kDa and 93 kDa (PMID: 9334170; 3171685).