NCAPD2 Polyclonal antibody, PBS Only

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• NCAPD2 Polyclonal antibody, PBS Only (13382-1-PBS)

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  WB analysis using 13382-1-AP (same clone as 13382-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 13382-1-AP (same clone as 13382-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 13382-1-AP (same clone as 13382-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13382-1-AP (same clone as 13382-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 13382-1-AP (same clone as 13382-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 13382-1-AP (same clone as 13382-1-PBS)

  IP result of anti-NCAPD2 (IP:13382-1-AP, 4ug; Detection:13382-1-AP 1:600) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 13382-1-AP (same clone as 13382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13382-1-AP (NCAPD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 13382-1-AP (same clone as 13382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 13382-1-AP (NCAPD2 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13382-1-AP (same clone as 13382-1-PBS)

  Immunofluorescent analysis of Hela cells, using NCAPD2 antibody 13382-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 13382-1-AP (same clone as 13382-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13382-1-AP (NCAPD2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NCAPD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 13382-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Condensin complex subunit 1, CNAP1, Chromosome-associated protein D2, Chromosome condensation-related SMC-associated protein 1, CAPD2

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Product Information

13382-1-PBS targets NCAPD2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4219

  Product name: Recombinant human NCAPD2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC028182

  Sequence: MAPQMYEFHLPLSPEELLKSGGVNQYVVQEVLSIKHLPPQLRAFQAAFRAQGPLAMLQHFDTIYSILHHFRSIDPGLKEDTLEFLIKVVSRHSQELPAILDDTTLSGSDRNAHLNALKMNCYALIRLLESFETMASQTNLVDLDLGGKGKKARTKAAHGFDWEEERQPILQLLTQLLQLDIRHLWNHSIIEEEFVSLVTGCCYRLLENPTINHQKNRPTREAITHLLGVALTRYNHMLSATVKIIQMLQHFEHLAPVLVAAVSLWATDYGMKSIVGEIVREIGQKCPQELSRDPSGTKGFAAFLTELAERVPAILMSSMCILLDHLDGENYMMRNAVLAAMAEMVLQVLSG

• Full Name: non-SMC condensin I complex, subunit D2
• Calculated Molecular Weight: 1401 aa, 157 kDa
• Observed Molecular Weight: 157 kDa
• GenBank Accession Number: BC028182
• Gene Symbol: NCAPD2
• Gene ID (NCBI): 9918
• RRID: AB_2149840
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15021
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NCAPD2 is a regulatory subunit of the condensin complex, which is required for conversion of interphase chromatin into mitotic-like condense chromosomes.  Condensin complexes are heteropentamers comprised of two SMC (structural maintenance of chromosomes) subunits and three non-SMC subunits. The C-terminus of NCAPD2 interacts with Histones H1 and H3 through their histone tails. A loss of NCAPD2 can lead to the disorganization of chromatid axes, misalignment of sister chromatids during metaphase and delayed entry into anaphase.