Cardinal Red™-conjugated NCL Polyclonal antibody

NCL Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

Cardinal Red™ Fluorescent Dye

Cat no : CR-10556

Synonyms

C23, FLJ45706, NCL, nucleolin, Protein C23

---

Validation Data Gallery

• 

  FC experiment of HeLa using CR-10556

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Cardinal Red™ Anti-Human NCL (CR-10556) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Product Information

CR-10556 targets NCL in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NCL fusion protein Ag0859
• Full Name: nucleolin
• Calculated molecular weight: 76 kDa
• Observed molecular weight: 100-110 kDa
• GenBank accession number: BC006494
• Gene symbol: NCL
• Gene ID (NCBI): 4691
• Conjugate: Cardinal Red™ Fluorescent Dye
• Excitation/Emission maxima wavelengths: 592 nm / 611 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation. Whilst mammalian nucleolin has a predicted molecular mass of approximately 77 kDa, the apparent molecular mass is between 100 and 110 kDa, and has been attributed to the amino acid composition of the N-terminal domain, which is highly phosphorylated. (PMID: 15925566)