Cardinal Red™-conjugated NCL Polyclonal antibody
NCL Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
Cardinal Red™ Fluorescent Dye
Cat no : CR-10556
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Sample-dependent, check data in validation data gallery |
Product Information
CR-10556 targets NCL in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NCL fusion protein Ag0859 |
Full Name | nucleolin |
Calculated molecular weight | 76 kDa |
Observed molecular weight | 100-110 kDa |
GenBank accession number | BC006494 |
Gene symbol | NCL |
Gene ID (NCBI) | 4691 |
Conjugate | Cardinal Red™ Fluorescent Dye |
Excitation/Emission maxima wavelengths | 592 nm / 611 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nucleolin, also known as C23, involved in the control of transcription of ribosomal RNA (rRNA) genes by RNA polymerase I, in nucleocytoplasmic transportation of ribosomal components, and in ribosome maturation and assembly. It associated with intranucleolar chromatin and pre-ribosomal particles, and induced chromatin decondensation by binding to histione H1. Also it has a role in the process of transcriptional elongation. Whilst mammalian nucleolin has a predicted molecular mass of approximately 77 kDa, the apparent molecular mass is between 100 and 110 kDa, and has been attributed to the amino acid composition of the N-terminal domain, which is highly phosphorylated. (PMID: 15925566)
Protocols
Product Specific Protocols | |
---|---|
FC protocol for Cardinal Red™ NCL antibody CR-10556 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |