Tested Applications
| Positive WB detected in | HeLa cells, human brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21177-1-AP targets Kiz in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15454 Product name: Recombinant human NCRNA00153 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 221-570 aa of BC045826 Sequence: PVSEYCESENKWSQEKHSPWEGVSDHLAHREPKSQKPFRKMQEEEEESWSTSSDLTISISEDDLILESPEPQPNPGGKMEGEDGIEALKLIHAEQERVALSTEKNCILQTLSSPDSEKESSTNAPTREPGQTPDSDVPRAQVGQHVATLKEHDNSVKEEATALLRKALTEECGRRSAIHSSESSCSLPSILNDNSGIKEAKPAVWLNSVPTREQEVSSGCGDKSKKENVAADIPITETEAYQLLKKATLQDNTNQTENRFQKTDASVSHLSGLNIGSGAFETKTANKIASEASFSSSEGSPLSRHENKKKPVINLKSNALWDESDDSNSEIEAALRPRNHNTDDSDDFYD Predict reactive species |
| Full Name | non-protein coding RNA 153 |
| Calculated Molecular Weight | 673 aa, 75 kDa |
| Observed Molecular Weight | 90-100 kDa |
| GenBank Accession Number | BC045826 |
| Gene Symbol | Kiz |
| Gene ID (NCBI) | 55857 |
| RRID | AB_10755292 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q2M2Z5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Kiz antibody 21177-1-AP | Download protocol |
| IHC protocol for Kiz antibody 21177-1-AP | Download protocol |
| IP protocol for Kiz antibody 21177-1-AP | Download protocol |
| WB protocol for Kiz antibody 21177-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Kiz polyclonal antibody (catalog number 21177-1-AP) has 5 total citations. These appear in three journals: Science, PLoS One, and eLife. The associated research fields center on cell biology and cytoskeletal organization, specifically acentrosomal spindle assembly in mammalian and human oocytes, maintenance of mitotic spindle bipolarity in cancer cells, and the hierarchical assembly of distal appendages that organize primary cilia. Applications cited include immunofluorescence (IF) and Western blot (WB).
| Species | Application | Title |
|---|---|---|
Science A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. | ||
PLoS One Two cancer cell lines utilize Myosin 10 and the kinesin HSET differentially to maintain mitotic spindle bipolarity | ||
Elife A hierarchical pathway for assembly of the distal appendages that organize primary cilia | ||
Elife Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendages |
Reviews
What customers say
The single review for 21177-1-AP is a 5-star rating for Immunofluorescence on HEK293 cells. The user stained for Kizuna and PCNT, using cold methanol fixation, Triton/Tween/SDS permeabilization, and 5% BSA blocking. The primary antibody was applied at 1:200 dilution for 1 hour at room temperature, with an Alexa-555 anti-rabbit secondary at 1:600. The protocol produced clear pericentriolar and DNA staining, indicating strong performance for IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Elisa (Verified Customer) (05-15-2022) | HEK293 cells stained for Hoechst (DNA marker, in green), Kizuna (in magenta) and PCNT (pericentriolar matrix marker, in green). HEK293 cells were plated on Poly-lysine coated coverslips and fixed in cold methanol for 5' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).
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