NEUROD1 Monoclonal antibody

NEUROD1 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3E10F1

Cat no : 66691-1-Ig

Synonyms

BETA2, BHF 1, bHLHa3, NEUROD, NEUROD1, neurogenic differentiation 1



Tested Applications

Positive WB detected inY79 cells, SH-SY5Y cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66691-1-Ig targets NEUROD1 in WB, IHC, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NEUROD1 fusion protein Ag27606
Full Name neurogenic differentiation 1
Calculated molecular weight 356 aa, 40 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC009046
Gene symbol NEUROD1
Gene ID (NCBI) 4760
RRIDAB_2882045
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. The basic helix-loop-helix (bHLH) proteins are transcription factors that are required for several aspects of development, including cell type determination, terminal differentiation and sex determination. Members of the myogenic determination family, MyoD, myf5, myogenin and MRF4, all have bHLH domains.These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG. Neuro D is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors and Neuro D can convert presumptive epidermal cells into neurons.The lack of NeuroD in the brain results in severe defects in development. Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young. The calculated molecular weight of NEUROD1 is 39 kDa, but the modified NEUROD1 protein is about 45-50 kDa.

Protocols

Product Specific Protocols
WB protocol for NEUROD1 antibody 66691-1-IgDownload protocol
IHC protocol for NEUROD1 antibody 66691-1-IgDownload protocol
FC protocol for NEUROD1 antibody 66691-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols