NEUROD1 Monoclonal antibody
NEUROD1 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3E10F1
Cat no : 66691-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Y79 cells, SH-SY5Y cells |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Product Information
66691-1-Ig targets NEUROD1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | NEUROD1 fusion protein Ag27606 |
Full Name | neurogenic differentiation 1 |
Calculated molecular weight | 356 aa, 40 kDa |
Observed molecular weight | 40-50 kDa |
GenBank accession number | BC009046 |
Gene symbol | NEUROD1 |
Gene ID (NCBI) | 4760 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. The basic helix-loop-helix (bHLH) proteins are transcription factors that are required for several aspects of development, including cell type determination, terminal differentiation and sex determination. Members of the myogenic determination family, MyoD, myf5, myogenin and MRF4, all have bHLH domains.These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG. Neuro D is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors and Neuro D can convert presumptive epidermal cells into neurons.The lack of NeuroD in the brain results in severe defects in development. Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young. The calculated molecular weight of NEUROD1 is 39 kDa, but the modified NEUROD1 protein is about 45-50 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for NEUROD1 antibody 66691-1-Ig | Download protocol |
IHC protocol for NEUROD1 antibody 66691-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |