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CoraLite®488-conjugated NEUROD1 Monoclonal antibody

NEUROD1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

3E10F1

Cat no : CL488-66691

Synonyms

BETA2, BHF 1, bHLHa3, NEUROD, NEUROD1, neurogenic differentiation 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite®488-conjugated NEUROD1 Monoclonal antibod (CL488-66691)

    FC experiment of SH-SY5Y using CL488-66691

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human NEUROD1 (CL488-66691, Clone:3E10F1) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66691 targets NEUROD1 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NEUROD1 fusion protein Ag27606
Full Name neurogenic differentiation 1
Calculated Molecular Weight 356 aa, 40 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC009046
Gene Symbol NEUROD1
Gene ID (NCBI) 4760
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths491 nm / 516 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. The basic helix-loop-helix (bHLH) proteins are transcription factors that are required for several aspects of development, including cell type determination, terminal differentiation and sex determination. Members of the myogenic determination family, MyoD, myf5, myogenin and MRF4, all have bHLH domains.These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG. Neuro D is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors and Neuro D can convert presumptive epidermal cells into neurons.The lack of NeuroD in the brain results in severe defects in development. Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young. The calculated molecular weight of NEUROD1 is 39 kDa, but the modified NEUROD1 protein is about 45-50 kDa.

Protocols

Product Specific Protocols
FC protocol for CL488 NEUROD1 antibody CL488-66691Download protocol
Standard Protocols
Click here to view our Standard Protocols