CoraLite® Plus 488-conjugated NFATC3 Monoclonal antibody

• 

  IF Staining of HepG2 using CL488-66924

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 NFATC3 antibody (CL488-66924, Clone: 1C1H8 ) at dilution of 1:200.

NFATC3 Monoclonal Antibody for IF/ICC

Cat No. CL488-66924

Clone No.1C1H8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

1C1H8, NF AT4, NF ATc3, NFAT4, NF-AT4

---

Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66924 targets NFATC3 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27306

  Product name: Recombinant human NFATC3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-205 aa of BC001050

  Sequence: MTTANCGAHDELDFKLVFGEDGAPAPPPPGSRPADLEPDDCASIYIFNVDPPPSTLTTPLCLPHHGLPSHSSVLSPSFQLQSHKNYEGTCEIPESKYSPLGGPKPFECPSIQITSISPNCHQELDAHEDDLQINDPEREFLERPSRDHLYLPLEPSYRESSLSPSPASSISSRSWFSDASSCESLSHIYDDVDSELNEAAARFTL

• Full Name: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
• Calculated Molecular Weight: 116 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC001050
• Gene Symbol: NFATC3
• Gene ID (NCBI): 4775
• RRID: AB_2934505
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q12968
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)