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CoraLite® Plus 488-conjugated NFS1 Monoclonal antibody

NFS1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1


Human, Mouse, Rat


IF, FC (Intra)


CoraLite® Plus 488 Fluorescent Dye



Cat no : CL488-67021



Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells

Recommended dilution

Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67021 targets NFS1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NFS1 fusion protein Ag8017
Full Name NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
Calculated Molecular Weight 50 kDa
GenBank Accession NumberBC018471
Gene Symbol NFS1
Gene ID (NCBI) 9054
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFS1(nitrogen fixation 1 homolog) is also named as NIFS, HUSSY-08 and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. The protein has been identified as a pyridoxal phosphate-containing homodimer that catalyzes the formation of equimolar amounts of elemental sulfur and L-alanine from the substrate, L-cysteine. It is reported that NFS1 is also able to catalyze the removal of selenium from selenocysteine, a mechanism similar to the L-cysteine reaction was postulated(PMID:9812986). It has 2 isoforms produced by alternative initiation.


Product Specific Protocols
IF protocol for CL Plus 488 NFS1 antibody CL488-67021Download protocol
FC protocol for CL Plus 488 NFS1 antibody CL488-67021Download protocol
Standard Protocols
Click here to view our Standard Protocols