NIT1 Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• NIT1 Polyclonal antibody, PBS Only (14380-1-PBS)

• 

  WB analysis using 14380-1-AP (same clone as 14380-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14380-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14380-1-AP (same clone as 14380-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14380-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293T using 14380-1-AP (same clone as 14380-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14380-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 14380-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 14380-1-AP (same clone as 14380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14380-1-AP (NIT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 14380-1-AP (same clone as 14380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14380-1-AP (NIT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 14380-1-AP (same clone as 14380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14380-1-AP (NIT1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 14380-1-AP (same clone as 14380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14380-1-AP (NIT1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14380-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14380-1-AP (same clone as 14380-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14380-1-AP (NIT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14380-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NIT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14380-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

---

Product Information

14380-1-PBS targets NIT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5764

  Product name: Recombinant human NIT1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-243 aa of BC046149

  Sequence: MLGFITRPPHRFLSLLCPGLRIPQLSVLCAQPRPRAMAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGPCLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEPVSS

• Full Name: nitrilase 1
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 32-36 kDa
• GenBank Accession Number: BC046149
• Gene Symbol: NIT1
• Gene ID (NCBI): 4817
• RRID: AB_2151314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86X76
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.