NKG2E Recombinant monoclonal antibody

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• NKG2E Recombinant monoclonal antibody (87818-1-RR)

• 

  WB analysis using 87818-1-RR

  Recombinant protein were subjected to SDS PAGE followed by western blot with 87818-1-RR (NKG2E antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  Affinity of 87818-1-RR

  Biolayer interferometry (BLI) kinetic assays of 87818-1-RR against Human NKG2E were performed. The affinity constant is 0.298 nM.

NKG2E Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87818-1-RR

Clone No.251269E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

KLRC3, NK cell receptor E, NKG2 E, NKG2 E activating NK receptor

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87818-1-RR targets NKG2E in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg4703

  Product name: recombinant human NKG2E protein

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 94-240 aa of NM_002261.3

  Sequence: IPFLEQNNSSPNARTQKARHCGHCPEEWITYSNSCYYIGKERRTWEESLQACASKNSSSLLCIDNEEEMKFLASILPSSWIGVFRNSSHHPWVTINGLAFKHEIKDSDHAERNCAMLHVRGLISDQCGSSRIIRRGFIMLTRLVLNS

• Full Name: killer cell lectin-like receptor subfamily C, member 3
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 45-60 kDa
• GenBank Accession Number: NM_002261.3
• Gene Symbol: KLRC3
• Gene ID (NCBI): 3823
• RRID: AB_3745768
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q07444-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NKG2E, also known as killer cell lectin-like receptor subfamily C member 3 (KLRC3), is a transmembrane protein primarily expressed on the surface of natural killer (NK) cells and some cytotoxic T-cells. As a member of the NKG2 family, it functions as a receptor for the recognition of non-classical MHC class I molecule HLA-E, playing a role in immune surveillance. Structurally, NKG2E is a type II membrane protein containing a C-type lectin domain in its extracellular region. The immunogenic sequence of NKG2E shares 88% homology with that of NKG2A, leading to cross-reactivity.