Anti-Human NKp46/NCR1 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98340-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human NKp46/NCR1 Rabbit RecAb (98340-1-RR, Clone: 242549D12) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98340-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human NKp46/NCR1 Rabbit RecAb (98340-1-RR, Clone: 242549D12) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

NKp46/NCR1 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98340-1-RR

Clone No.242549D12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

NCR 1, Natural killer cell p46-related protein, LY94, hNKp46, CD335

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98340-1-RR targets NKp46/NCR1 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: natural cytotoxicity triggering receptor 1
• Calculated Molecular Weight: 34kDa
• GenBank Accession Number: /
• Gene Symbol: NCR1
• Gene ID (NCBI): 9437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O76036-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).