CoraLite® Plus 647 Anti-Human NKp46/NCR1 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using CL647-98340

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human NCAM1/CD56, and 5 ul CoraLite® Plus 647 Anti-Human NKp46/NCR1 Rabbit RecAb (CL647-98340, Clone: 242549D12) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL647-98340

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human NKp46/NCR1 Rabbit RecAb (CL647-98340, Clone: 242549D12) (red), or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

NKp46/NCR1 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98340

Clone No.242549D12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

242549D12, CD335, hNKp46, LY94, Natural killer cell p46-related protein

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98340 targets NKp46/NCR1 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NKp46/NCR1 fusion protein Eg1364
• Full Name: natural cytotoxicity triggering receptor 1
• Calculated Molecular Weight: 34kDa
• Gene Symbol: NCR1
• Gene ID (NCBI): 9437
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O76036-1
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).