NME1/2 Recombinant antibody, PBS Only

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  WB analysis using 85074-2-RR (same clone as 85074-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85074-2-RR (NME1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85074-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293T using 85074-2-RR (same clone as 85074-2-PBS)

  1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug NME1/2 Recombinant antibody (85074-2-RR, Clone:242698C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85074-2-PBS in a different storage buffer formulation.

NME1/2 Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 85074-2-PBS

Clone No.242698C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

NME2, 242698C7, C-myc purine-binding transcription factor PUF, EC:2.7.13.3, EC:2.7.4.6

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Product Information

85074-2-PBS targets NME1/2 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NME1/2 fusion protein Ag13913
• Full Name: non-metastatic cells 2, protein (NM23B) expressed in
• Calculated Molecular Weight: 152 aa, 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC002476
• Gene Symbol: NME2
• Gene ID (NCBI): 4831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P22392
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NME2(non-metastatic cells 2, protein) is also named as NDKB(Nucleoside diphosphate kinase B), NM23B and belongs to the NDK family. It is involved in the phosphorylation of nucleoside diphosphates and interacts with membrane receptor and constituting a novel molecular regulatory mechanism of GPCR endocytosis. This protein has 2 isoforms produced by alternative splicing. Refer to epitope and protein detection, the antibody 85074-2-RR detects both NME1& NME2.