NQO2 Polyclonal antibody, PBS Only

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• NQO2 Polyclonal antibody, PBS Only (15767-1-PBS)

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  WB analysis using 15767-1-AP (same clone as 15767-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15767-1-AP (NQO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 15767-1-AP (same clone as 15767-1-PBS)

  IP result of anti-NQO2 (IP:15767-1-AP, 3ug; Detection:15767-1-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 15767-1-AP (same clone as 15767-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15767-1-AP (NQO2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 15767-1-AP (same clone as 15767-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15767-1-AP (NQO2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15767-1-AP (same clone as 15767-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NQO2 antibody (15767-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NQO2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 15767-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

15767-1-PBS targets NQO2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8403

  Product name: Recombinant human NQO2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-231 aa of BC006096

  Sequence: MAGKKVLIVYAHQEPKSFNGSLKNVAVDELSRQGCTVTVSDLYAMNFEPRATDKDITGTLSNPEVFNYGVETHEAYKQRSLASDITDEQKKVREADLVIFQFPLYWFSVPAILKGWMDRVLCQGFAFDIPGFYDSGLLQGKLALLSVTTGGTAEMYTKTGVNGDSRYFLWPLQHGTLHFCGFKVLAPQISFAPEIASEEERKGMVAAWSQRLQTIWKEEPIPCTAHWHFGQ

• Full Name: NAD(P)H dehydrogenase, quinone 2
• Calculated Molecular Weight: 231 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC006096
• Gene Symbol: NQO2
• Gene ID (NCBI): 4835
• RRID: AB_2236213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16083
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NQO2, also named as QR2 and NMOR2, belongs to the NAD(P)H dehydrogenase (quinone) family. It serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. The cytosolic quinone oxidoreductases NQO1 and NQO2 protect cells against oxidative stress by detoxifying quinones and preventing redox cycling. NQO1 and NQO2 are important endogenous factors in regulation of immune response and autoimmunity.