NRCAM Monoclonal antibody

NRCAM Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2E1C3

Cat no : 68291-1-Ig

Synonyms

hBravo, KIAA0343, Neuronal surface protein Bravo, Ng CAM related, Nr CAM, NRCAM

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Validation Data Gallery

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  WB analysis using 68291-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68291-1-Ig (NRCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat cerebellum using 68291-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68291-1-Ig (NRCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 68291-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68291-1-Ig (NRCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 68291-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NRCAM antibody (68291-1-Ig, Clone: 2E1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: rat brain tissue, pig brain tissue, rat cerebellum tissue, mouse brain tissue, pig cerebellum tissue
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68291-1-Ig targets NRCAM in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: NRCAM fusion protein Ag20558
• Full Name: neuronal cell adhesion molecule
• Calculated Molecular Weight: 1304 aa, 144 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC098401
• Gene Symbol: NRCAM
• Gene ID (NCBI): 4897
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuronal cell adhesion molecule (NRCAM) is a member of the L1 subfamily of cell adhesion molecules (CAMs) that belong to the immunoglobulin superfamily (PMID: 11329126). NRCAM is a transmembrane protein composed of six Ig-like domains and five fibronectin type-III repeats in the extracellular region, with a highly conserved cytoplasmic tail. It is mainly expressed in the nervous system and is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. NRCAM is also expressed outside the nervous system. Altered expression of NRCAM has been associated with tumor progression in diverse organs (PMID: 22182708).