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CoraLite® Plus 488-conjugated NRF2, NFE2L2 Recombinant antibody

NRF2, NFE2L2 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1I21

Cat no : CL488-80593

Synonyms

HEBP1, NF E2 related factor 2, NFE2 related factor 2, NFE2L2, NRF2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated NRF2, NFE2L2 Recombi (CL488-80593)

    FC experiment of MCF-7 using CL488-80593

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human NRF2, NFE2L2 (CL488-80593, Clone:1I21) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-80593 targets NRF2, NFE2L2 in FC (Intra) applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NRF2, NFE2L2 fusion protein Ag9489
Full Name nuclear factor (erythroid-derived 2)-like 2
Calculated Molecular Weight 605 aa, 68 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC011558
Gene Symbol NFE2L2
Gene ID (NCBI) 4780
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRF2, also named as NFE2L2, belongs to the bZIP family and CNC subfamily. It is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. NRF2 is important for the coordinated up-regulation of genes in response to oxidative stress. It may be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Nrf2 is a key player in the regulation of genes encoding for many antioxidative response enzymes.The expression of NRF2 may be induced under oxidative stress (PMID:14567983).In lung cancer, Nrf2 activation in malignant cells has been associated with tumor progression and chemotherapy resistance(PMID:20534738). Identifying patients with abnormal NRF2 expression may be important for selection for chemotherapy in NSCLC. As new investigators break into the emerging field of Nrf2 research, confusion regarding the correct migratory pattern of Nrf2 is causing doubts about the accuracy and reproducibility of published results. This letter provides solid evidence that the actually observed molecular weight of Nrf2 is about 70kDa and 95-110 kDa. (PMID: 22703241).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 NRF2, NFE2L2 antibody CL488-80593Download protocol
Standard Protocols
Click here to view our Standard Protocols